Spectroscopic ellipsometry (SE)
A label-free technique, SE is used to quantify the density and interaction stoichiometries of biomolecules on surfaces
SE measures changes in the polarisation of light when reflected off a material surface. From such data, the optical properties and thickness of thin films can be inferred.
In solvated biomolecular films, SE can be used to quantify the surface density of biomacromolecules. Interactions with biomolecular films can be monitored in real time and without the need for labels, enabling quantification of binding kinetics and stoichiometries.
Applications in our research
Spectroscopic ellipsometry is applied in the Richter lab to quantify the surface density of biomolecules, the kinetics and stoichiometry of biomolecular interactions, and the thickness of biomolecular films. Applications span across several current research projects; to learn more, please click any of the below icons.
